Natalie Lander

Krissy Holdout (voice)

Kate Micucci

Velma Dinkley (voice)

Matthew Lillard

Shaggy Rogers (voice)

Grey DeLisle

Daphne Blake (voice)

Iqbal Theba

Dr. Najib (voice)

Frank Welker

Scooby-Doo / Fred Jones (voice)

Kate Higgins

Brenda (voice)

Hynden Walch

Mitzi Capaletto (voice)

Jeff Bennett

Deputy / Museum Guide (voice)

Kevin Michael Richardson

Sheriff (voice)